MI 822

Immunity, Infection, and Disease


 

 


IID Laboratory Manual

 

                                                                             

Robert J. Jacob, Ph.D.    l    Jan M.Thompson, Ph.D.

Course Director                    Laboratory Director

 

 

 

Acknowledgments

 

Information used in this version was taken from previous IID laboratory manuals, the Tenth Edition of Bailey & Scott’s Diagnostic Microbiology, and the Seventh Edition of Manual of Clinical Microbiology.   Additionally, we wish to thank Linda Gilleland for permission to use information from her LSU Medical Center - Shreveport Laboratory Manual in the various versions of this laboratory manual.

 

Department of Microbiology and Immunology

College of Medicine

University of Kentucky

 

Ó2002

TABLE OF CONTENTS

 

                           Section

Section

Introduction to the Microbiology Laboratory

Common Laboratory Techniques

Laboratory 1  (9 AM - 12 N, Mon. 9/9/02)

Check In

Colony Isolation, Gram Stain, and Microscopy

 

Lab 1 Demonstration Images

Laboratory 2  (8 AM - 12 N, Wed. 911/02)

Conclusions to Laboratory 1

Gram-Positive Pathogens of Mucous Membranes

 

Lab 2 Demonstration Images

 

Laboratory 3  (10 AM - 12 N, Fri. 9/13/02)

            Conclusions to Laboratory 2

Gram-Negative Pathogens of Mucous Membranes

 

Lab 3 Demonstration Images

Laboratory 4  (10 AM - 12 N, Wed. 9/25/02)

Acid-Fast Respiratory Pathogens

Corynebacterium diphtheriae

 

Lab 4 Demonstration Images

 

Laboratory 5  (9 AM - 12 N, Fri. 10/4/02)

Medical Mycology

 

 

Lab 5 Demonstration Images

Laboratory 6  (8 AM - 12 N, Wed. 10/9/02) 

Gram-Negative Bacilli, UTIs

Case-based Unknowns No. 1, 2, and 3

 

Lab 6 Demonstration Images

 

Laboratory 7  (9 AM - 12 N, Fri. 10/11/02)

Conclusions to Laboratory 6

Case-based Unknowns No. 1, 2, and 3

Laboratory 8  (9 AM - 12 N, Mon. 10/14/02)

Case-based Unknowns No. 1, 2, and 3

 

Laboratory 9  (9 AM - 12 N, Wed. 10/16/02)

Case-based Unknowns No. 1, 2, and 3

Laboratory 10 (9 AM-12 N, Fri. 10/18/02)

Case-based Unknowns No. 1, 2, and 3

Turn in Results sheets for Unknowns

Check out

 

***Laboratory Practical  (9-11 AM, Mon. 10/28/02)***

Appendix 1:  The Bacteria

Appendix 2: Culture Media, Biochemical Tests, and Techniques